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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in many tumors, which leads to chromosome instability, and so might contribute to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential role in advertising cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence info is just not however out there.
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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded from the antibody.
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This guide outlines additional probable optimization of mobile and immunoassay parameters to make certain the very best results are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is precise.
Focus on Data Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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